%0 Journal Article
%T Antimicrobial Activity of the Istranca Oak (Quercus hartwissiana Steven) against Nosocomial Pathogens
%A £¿zge K£¿l£¿n£¿el
%J -
%D 2018
%X Aim: Recently, the resistance of pathogenic microorganisms due to the unconscious use of antibiotics has caused new antibiotic searches. In this study, it was aimed to determine the antimicrobial activity of £¿stranca oak (Quercus hartw£¿ss£¿ana Steven) extracts against bacterial and fungal pathogens. Materials and Methods: The plant was extracted with ethanol, formaldehyde, acetone, ethyl acetate and methanol solvents. After taking 50 ¦Ìl of these extracts and injecting them into the sterile discs, the discs were dried in sterile environment for 24 h in order not to solvents present in the extracts affect the result. Antimicrobial activity was researched by disc diffusion method against Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Salmonella spp., methicillin resistant Staphylococcus aureus, methicillin susceptible Staphylococcus aureus (MSSA), Staphylococcus epidermidis, Enterococcus spp., Candida albicans, Candida glabrata and Candida tropicalis species. Results: In the study, it was determined that the extract prepared with ethanol showed the widest zone diameter against Enterococcus spp. (8mm) and S.epidermidis (8mm); extracts prepared with formaldehyde and ethyl acetate showed the widest zone diameter against E.coli (16mm,28mm); extract prepared with acetone showed the widest zone diameter against Enterococcus spp. (21mm); extract prepared with methanol showed the widest zone diameter against MSSA (28mm). Among the yeasts; extract prepared with ethanol showed the widest zone diameter against C.glabrata (12mm); extract prepared with formaldehyde showed the widest zone diameter against C.albicans (28mm) and C.tropicalis (28mm); extracts prepared with acetone, ethyl acetate and methanol showed the widest zone diameter against C.albicans (19mm,30mm,22mm). Discussion and Conclusion: The extract prepared with ethyl acetate of the plant had higher antimicrobial activity than the others. In addition, it is thought that it is important to conduct in vivo antimicrobial studies on the antimicrobial effect of the naturally grown in our country because of the wide zone diameter of £¿stranca oak was found to be against E.coli, S.aureus and C.albicans strains
%K antimikrobiyal aktivite
%K Istranca me£¿esi
%K Quercus hartwissiana Steven
%U http://dergipark.org.tr/anadoluklin/issue/39354/412855