%0 Journal Article
%T The Effects of Physical and Chemical Conditions on Viral DNA Obtained by Various Methods
%A Zeynep AKKUTAY YOLDAR
%J -
%D 2018
%X DNA viruses are more resistant to environmental conditions than RNA viruses due to their repair mechanisms and stablity of their genetic material. Despite these advantages, inadequate laboratory conditions and inhibitory agents can interfere with viral DNA detection. In this study, which compares various methods of viral DNA obtainment, the effects of different physical and chemical conditions on viral nucleic acid were also questioned. For this purpose, a total of four different extraction methods, one of which was the conventional method, have been used to detect the DNA of the parapox virus causing ecythma contagiosum. The isolated DNAs were exposed to various physical (pressure, uv) and chemical treatment (detergent) conditions before PCR amplification. While viral DNA amplification occured after exposure to autoclave pressure steam sterilizer; Tween 20 and UV radiation inhibited viral DNA amplification. While most commercial kits helped to shorten the duration of DNA extraction, the conventional method provided the highest efficiency for viral DNA detection
%K Ekstraksiyon
%K PCR
%K Viral DNA
%U http://dergipark.org.tr/huvfd/issue/42355/508964