%0 Journal Article
%T Antibacterial Activity of Muscid and Myiasis Flies Larval Secretions
%A AYKUT ZEREK
%J -
%D 2019
%X Maggot therapy is a method based on the use of myiasisfly larvae also called maggot which affinity necrotic tissue in wound treatment. In this study, it was aimed to determine the antibacterial activity some muscid and myiasis flies larvae secretions in vitro. Investigation of the effects of antibacterial compounds produced by the fly larvae secretions have become important with the increasing of antibacterial-resistant bacteria strains. In this study, Lucilia sericata, Chrysomya albiceps, Calliphora vicinaand Musca domesticaflies catched and placed in the cage to provide feeding and laying. After disinfected the eggs was transferred to sterile medium including sheep blood agar (5 %) and incubated overnight at 25 ¡ãC. After hatched, larvae were kept in the medium for 2-3 days and disinfected, incubated overnight at 25 ¡ãC in steril bi-distilled water (ddH2O). After incubation, the liquid which containing larval secretions, centrifuged and sterilized by filtration (0.2 ¦Ìm) that bacteria could not pass through. Staphylococcus aureus, MRSA ATCC 43300, E. coliand P. aeruginosathe suspension of bacteria spreaded petri dishes including MHA sterile-medium. Secretions amount of 30 ¦Ìl obtained from larvae was impregnated blank antibiotic discs and were placed in the MHA medium and then incubated overnight at 35 ¡ãC.When the results were examined, antibacterial activitywere not detected againstGram-negative E. coliand P. aeruginosabacterial strainsat thelarval secretions of muscid and myiasis flies, antibacterial activitywas detected against Gram-positive S. aureusbacterial strain. While antibacterial activity was not detected against Gram-positive MRSA ATCC 43300 bacterial strain the larval secretion of muscidfly,antibacterial activity were detected the larval secretions of myiasis flies.In conclusion, the antibacterial activities of muscid and myiasis fly larval secretionswere showed and it is suggested that studies regarding this field can be investigated by using different method and medium.
%K Maggot terapi
%K Muskid ve miyaz
%K Larva
%K Salg£¿
%K Antibakteriyel etki
%U http://dergipark.org.tr/vanvetj/issue/47335/441028