%0 Journal Article
%T Sugar compositions by HPLC of asphodel (Asphodelus aestivus Brot.) tuber with enzymatic, acidic and water extraction methods
%A Eyy¨¹p KARAO£¿UL
%J -
%D 2018
%X The aim of this study was to investigate extraction yield and soluble sugar composition of asphodel (Asphodelus aestivus Brot.) tubers. The tubers of asphodel were extracted with enzymatic extraction methods (150 ¦ÌL, 300 ¦ÌL and 450 ¦ÌL added Gluko-Amilaz, ¦Á-Amilaz and simultaneously ¦Á-Amilaz & Gluko-Amilaz enzyme), deionized water extraction methods (60 min, 90 min, 120 min and 180 min extraction time) and acidic extraction methods (0,8 M and 1,6 M concentration with acetic and sulfuric acid). The sugar compositions of tubers were determined by high performance liquid chromatography and refractive index detector (HPLC-RID). The extraction yields were also detected by gravimetric method. The glucose, galactose, xylose, fructose and sucrose were used as reference compounds. The calibration curves were plotted based on linear regression analysis of the integrated peak areas (x) and versus concentrations (y, ¦Ìg/ml) in the reference solutions. All results were evaluated as concentration (¦Ìg/ml) for every extraction method. Various types of extraction caused differences in both yields and sugar units. Generally, extraction yields were determined about 81-96% for all extraction methods. However, the highest extraction yield was found 96% for deionized water unlike the ones added enzyme or acid. At the same time, results showed that glucose, fructose, mannose and sucrose were found in tubers in all methods. The main concentrations of sugar in the tuber were identified as 2.510 ¦Ìg/ml for sucrose in enzymatic method, 50.144 ¦Ìg/ml for glucose, 21.066 ¦Ìg/ml for fructose, 1.043 ¦Ìg/ml for xylose and 2.180 ¦Ìg/ml for galactose in acidic methods. Nevertheless, the lowest amount of glucose and fructose were found in deionized water extraction method at 180 min. The results indicated that sucrose amount had higher concentration in some extraction methods due to hydrolysis of carbohydrates in oligomer or polymer form. Moreover, glucose and fructose were also determined higher amounts in some extraction methods due to hydrolysis of sucrose. It can be understood that when the extraction conditions are more extreme, all sugar unit may convert to hydroxymethylfurfural
%K £¿iri£¿ otu (Asphodelus aestivus Brot.) Yumrular£¿
%K Ekstraksiyon metotlar£¿
%K Ekstraksiyon Verimleri
%K HPLC-RID
%K £¿eker kompozisyonlar£¿
%U http://dergipark.org.tr/ogmoad/issue/33136/401318