%0 Journal Article
%T LEPTIN QUANTITY DECREASES £¿N TYPE 1 DIABETES MELLITUS INDUCUED RATS BRAIN
%A Erhan £¿AH£¿N
%J -
%D 2019
%X OBJECTIVE: Leptin hormone plays an important role in regulation of appetite and body metabolism and is mainly synthesized in fat tissue. It is known that leptin is transported to the brain via choroid plexus after synthesized in the fat tissue. The aim of this study is to investigate the amount and expression of leptin in the brain of rats with type 1 diabetes mellitus. MATERIAL AND METHODS: A total of 14 adult male Wistar Albino rats were divided into 2 equal groups (n = 7). No application was made to the control group. A single dose (55 mg/kg) of streptozotocin was administered intraperitoneally to the animals of the diabetes mellitus group and it was considered that type 1 diabetes mellitus developed when blood glucose level > 280 mg/dL. Brain samples taken at the end of the experiment were fixed with %10 formaldehyde and then subjected to routine tissue monitoring followed by leptin immunohistochemical staining. The amount of leptin was determined by western blotting in brain tissues. RESULTS: In all groups leptin staining was detected only in the choroid plexus of rat brains. According to this knowlodge, it was determined that leptin staining decreased in type 1 diabetes mellitus developed rats. The decreased leptin in the type 1 diabetes mellitus group was showed by western blot too. CONCLUSIONS: Type 1 diabetes reduces the expression and amount of leptin in the choroid plexus
%K Diyabetes mellitus
%K beyin
%K leptin
%K koroid pleksus
%U http://dergipark.org.tr/kocatepetip/issue/44892/408118