%0 Journal Article
%T ER, SOCS1, p15, E-cadherin and RARB are more Likely to be Members of the Methylator Phenotype of Adult and Childhood AML and Their Methylation is Primarly Regulated by an Overexpression of DNMT 3A
%A Cumhur G. Ekmek£¿i
%J -
%D 2018
%X DOI: 10.26650/experimed.2018. 473615 Objectives: The hypermethylator phenotype is used as a term for both acute myeloid leukemia (AML) and other tumors. We analyzed the methylation statuses of ten genes (DAP-kinase, SOCS1, ER, p15, Ecadherin, RAR¦Â, p16, GSTP1, HIC1 and p73) and correlated them with the expression of DNA methyltransferases (DNMTs). Material and Method: We analyzed the methylation profiles using methylation specific PCR and COBRA and the expression of DNMTs (DNMT 1, 3A and 3B) by quantitative RT-PCR in 25 pediatric and 25 adult AML samples. Results: The ER, SOCS1, p15, E cadherin, and RARB genes methylated together significantly in the same patients and members of the methylator phenotype. Conclusion: Our study demonstrated that the gene DNMT3A was dramatically upregulated and significantly correlated with the methylator phenotype
%K Metilat£¿r fenotipi
%K MSP
%K epigenetik
%K t¨¹m£¿r bask£¿lay£¿c£¿ genler
%K akut miyeloid l£¿semi
%K DNA metiltransferazlar
%U http://dergipark.org.tr/experimed/issue/43462/473615