%0 Journal Article
%T Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution
%A Chusnul Hidayat
%A Indro Prastowo
%A Pudji Hastuti
%J -
%D 2015
%R 10.9767/bcrec.10.3.8511.230-236
%X A fed-batch system was modified for the enzymatic production of Oleic Acid Ethyl Ester (OAEE) using rice bran (Oryza sativa) lipase by retaining the substrate molar ratio (ethanol/oleic acid) at 2.05:1 during the reaction. It resulted in an increase in the ester conversion of up to 76.8% in the first 6 h of the reaction, which was then followed by a decrease from 76.8% to 22.9% in 6 h later. The production of water in the reaction system also showed a similar trend. The water was hypothesized to lead lipase to reverse the reaction which resulted in a decrease in both (water and esters) in the last 6 h of the reac- tion. In order to overcome the problem, zeolite powder (25 and 50 mg/mL) were added into the reaction system at 5 h of the reaction. As the result, the final ester conversions increased drastically up to 90 - 95.7%. Thus, the combination of a constant substrate molar ratio (ethanol/oleic acid) during the reaction (at 2.05:1) with the addition of zeolite powder (25 and 50 mg/mL) to the reaction system at 5 h is effective for the enzymatic synthesis of OAEE. £¿ 2015 BCREC UNDIP. All rights reserved Received: 2nd May 2015; Revised: 20th June 2015; Accepted: 2nd July 2015 How to Cite: Prastowo, I., Hidayat, C., Hastuti, P. (2015). Production of Oleic Acid Ethyl Ester Catalyzed by Crude Rice Bran (Oryza sativa) Lipase in a Modified Fed-batch System: A Problem and its Solution. Bulletin of Chemical Reaction Engineering & Catalysis, 10 (3): 230-236. (doi:10.9767/bcrec.10.3.8511.230-236) Permalink/DOI: http://dx.doi.org/10.9767/bcrec.10.3.8511.230-23
%K Fed-batch system
%K rice bran (Oryza sativa) lipase
%K substrate molar ratio (ethanol/oleic acid)
%K water
%K zeolite powder
%U https://ejournal.undip.ac.id/index.php/bcrec/article/view/8511