%0 Journal Article %T Novel variant in the leptin receptor (LEPR) gene and its association with fat quality, odour and flavour in sheep %A A. Gunawan %A C. Sumantri %A F. W. Pramukti %A I. Inounu %A J. Jakaria %A K. Listyarini %A M. A. Abuzahra %A M. J. Uddin %J - %D 2019 %R 10.14710/jitaa.44.1.1-9 %X The aim of this study was to investigate the association and expression of LEPR with fat quality, flavour and odour in sheep. A total of 47 rams were used in this study. Fatty acids composition, BCFA (Branch Chanin Fatty Acids; MNA, MP, MOA, and EOA) and skatole (MI) representing fat quality, flavour and odour in sheep were measured at ages of 10-12 months old. Identification of novel variant and expression analysis of LEPR gene with different fat quality, odour and flavour were performed by PCR RFLP and qRT-PCR, respectively. A SNP in genomic region g.40854778 A>C of the LEPR gene was associated (P<0.05) with fat quality of saturated fatty acids (SFA) including [tricosanoic acid (C23:0) and tetracosanoic acid (C24:0)] and poly unsaturated fatty acid (PUFA) [docosahexaenoic acid (C22:6n3)]. However, the novel variant of LEPR was not significantly associated (P<0.05) with fat content, odour and flavour (MNA, MP, MOA, EOA, and MI). The mRNA expression analysis showed that LEPR mRNA expression was higher (P<0.01) in sheep found at the AA genotype. It could be concluded that variant SNP in the LEPR may contribute to fatty acid composition without influencing odour and flavour traits in sheep %K flavour %K LEPR %K mRNA expression %K odour %K variant %U https://ejournal.undip.ac.id/index.php/jitaa/article/view/19465