%0 Journal Article
%T The Effect of Curcumin on Regression of Liver Fibrosis through Decreased Expression of Transforming Growth Factor-¦Â1 (TGF-¦Â1)
%A Asri Nugraheni
%A Handono Kalim
%A Mudjiwijono Handaru Eko
%A Supriono Supriono
%J -
%D 2019
%R https://doi.org/10.18585/inabj.v11i1.463
%X BACKGROUND: Transforming growth factor (TGF)-¦Â1 has a pivotal role in liver fibrogenesis. Curcumin effectively prevent the progression of liver fibrosis through inhibition of TGF-¦Â1/Sma and drosophila MAD (Smad) signaling pathway. However, the role of curcumin in the regression of liver fibrosis is still unknown. This study investigated the role of curcumin and TGF-¦Â1 in liver fibrosis regression. METHODS: An experimental Wistar rat model included 6 treatment groups as well as positive and negative control groups. The treatment and positive control groups were injected with carbon tetrachlorid (CCl4) for 9 weeks to induce liver fibrosis. After cessation of injection, 3 of the treatment groups were given curcumin and 3 were given carboxymethylcellulose (CMC) for 2, 5 and 9 weeks, while the positive control was untreated. The negative control was injected with normal saline. TGF-¦Â1 liver tissue levels were analyzed by ELISA, while the TGF-¦Â1 expression in liver cells was analyzed by immunohistochemical assay. The metavir score was used to assess the degree of liver fibrosis. Values of p<0.05 were regarded as statistically significant. RESULTS: Nine weeks of CCl4 injection induced liver fibrosis (metavir F3); and significantly increased TGF-¦Â1 levels and expression in tissues (p=0.00, p=0.021, respectively). Curcumin administration decreased levels and expression of TGF-¦Â1 in the liver and accelerated regression of liver fibrosis. There was a significant correlation between duration of administration of curcumin with an expression of TGF-¦Â1 in the liver tissue (r=0.87; p<0.00). CONCLUSION: Curcumin accelerates regression of liver fibrosis, likely through decreasing of TGF-¦Â1 expression in the liver. KEYWORDS: curcumin, TGF-¦Â1, liver fibrosis regression, CCl4, animal mode
%K curcumin
%K TGF-¦Â1
%K liver fibrosis regression
%K CCl4
%K animal model
%U https://inabj.org/index.php/ibj/article/view/463