%0 Journal Article
%T Inhibition activity of Robusta coffee beans polyphenol extract on the production of TNF-¦Á neutrophil cells
%A Happy Harmono
%A Tantin Ermawati
%A Zahara Meilawaty
%J -
%D 2018
%R https://doi.org/10.22146/majkedgiind.28352 https://doi.org/10.22146/majkedgiind.28352
%X Polyphenols are one of the active substances in the Robusta coffee beans with various benefits for humans¡¯ health including anti-inflammation. neutrophil cell (polymorphonuclear PMN) plays a significant role as the primary immune response against foreign agent. Inflammatory response is characterized by the production of pro-inflammatory cytokines such as tumor necrosis factor-¦Á (TNF-¦Á). Hence, the purpose of this study is to determine anti-inflammation capacity of Robusta coffee beans polyphenols extract on the TNF-¦Á production in neutrophil cells. Neutrophil was derived from human peripheral venous blood by means of gradient density method. One hundred microliter of neutrophil (1,5x103 cell) was incubated with 3.13%, 6.25%, 12.5%, 25% polyphenol extract and exposed with 100 ¦Ìl of Lipopolysaccharides (LPS) 25 mg/ml. The production of TNF-¦Á were determined by ELISA. Data were analyzed by one way Anova. Polyphenols of Robusta coffee bean extract was shown to inhibit TNF-¦Á production in LPS-exposed neutrophil cells compared to controls. Whereas, LPS-exposed neutrophil increased TNF-¦Á production. The most effective concentration to inhibit TNF-¦Á production was 12.5%. It was concluded that polyphenols of Robusta coffee beans has anti-inflammatory properties as indicated by its ability to decrease TNF-¦Á levels
%K LPS
%K neutrophil
%K polyphenol
%K Robusta coffee beans
%K TNF-¦Á
%U https://jurnal.ugm.ac.id/mkgi/article/view/28352