%0 Journal Article
%T An Enzyme-Free ¡°ON-OFF¡± Electrochemiluminescence Biosensor for Ultrasensitive Detection of PML/RAR¦Á based on Target-Switched DNA Nanotweezer
%J -
%D 2019
%R https://doi.org/10.1021/acsami.8b18497
%X Herein, an enzyme-free ¡°ON-OFF¡± electrochemiluminescence (ECL) biosensor for ultrasensitive detection of fusion gene PML/RAR¦Á is constructed based on a simple target-switched DNA nanotweezer as hemin concentration controller. In this biosensor, the hemin concentration is primarily controlled by the conversion of ¡°opened-closed¡± DNA nanotweezers and low concentration hemin is first used as electrochemically regenerable enhancer. In the absence of the target, the nanotweezers are in an opened state which lead to a low concentration of hemin in the solution, resulting in an enhanced Ru(bpy)32+ ECL signal. In the presence of the target, the closed nanotweezers absorbed onto the surface of electrode can capture the hemin, which achieves a high concentration of hemin and then quenches the ECL signal. The developed method achieves ultrasensitive detection of PML/RAR¦Á with a wide linear range from 1 fM to 1 nM and limit of detection as low as 0.125 fM. In addition, the ECL biosensor shows excellent specificity to the other subtypes of PML/RAR¦Á (subtype ¡°S¡±, ¡°V¡±), ¡°PML¡±, and ¡°RAR¦Á¡±. Moreover, due to the high designable character of DNA nanotweezer, this method might provide a pragmatic Ru(bpy)32+ ECL platform for ultrasensitive detection of nucleic acid in the future
%U https://pubs.acs.org/doi/10.1021/acsami.8b18497