%0 Journal Article
%T Cathepsin G Activity as a New Marker for Detecting Airway Inflammation by Microscopy and Flow Cytometry
%J -
%D 2019
%R https://doi.org/10.1021/acscentsci.8b00933
%X High Resolution Image Download MS PowerPoint Slide Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into the airways. To assess if cathepsin G (CG) is a novel key player in chronic lung inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The probes quantitatively revealed elevated CG activity in samples from 46 patients. For future basic science and personalized clinical applications, we developed a rapid, highly informative, and easily translatable small-molecule FRET flow cytometry assay for monitoring protease activity including cathepsin G. We demonstrated that mSAM distinguished healthy from patient cells by FRET-based flow cytometry with excellent correlation to confocal microscopy data
%U https://pubs.acs.org/doi/10.1021/acscentsci.8b00933