%0 Journal Article
%T Cascade Amplifiers of Intracellular Reactive Oxygen Species Based on Mitochondria-Targeted Core¨CShell [email protected]/H Nanorods for Breast Cancer Therapy
%J -
%D 2018
%R https://doi.org/10.1021/acsami.8b12590
%X Tumor cells are vulnerable to reactive oxygen species (ROS). However, it is still a challenge to induce ROS efficiently in tumor cells. In this study, cascade amplifiers of intracellular ROS based on charge-reversible mitochondria-targeted [email protected]/H nanorods (NRs) were first developed for breast cancer therapy. The core¨Cshell [email protected]/H NR with a particle size of 179.60 ¡À 5.67 nm was composed of a core of a ZnO NR, an inner shell of triphenyl phosphonium (TPP), and an outer shell of heparin. Doxorubicin (DOX) was loaded on [email protected]/H NRs with high drug loading efficiency of 22.00 ¡À 0.18%. The zeta potential of [email protected]/H NRs varied from 24.00 ¡À 0.83 to £¿34.06 ¡À 0.87 mV after heparin coating, protecting [email protected]/H NRs from nonspecific adsorption in circulation. Mitochondrial targeting was achieved after the degradation of heparin. Cellular uptake assays showed that [email protected]/H NRs could accumulate in mitochondria. ROS generation assays showed that [email protected]/H NRs could triple the intracellular ROS in 4T1 cells (highly metastatic breast cancer cells) than free DOX. Western blot demonstrated that [email protected]/H NRs dramatically induced cell apoptosis in 4T1 cells. In vivo experiments suggested the antitumor potential of [email protected]/H NRs
%U https://pubs.acs.org/doi/10.1021/acsami.8b12590