%0 Journal Article
%T DNA¨CNanoparticle Composites Synergistically Enhance Organophosphate Hydrolase Enzymatic Activity
%J -
%D 2018
%R https://doi.org/10.1021/acsanm.8b00933
%X Cell-free synthetic biology relies on optimally exploiting enzymatic activity, and recent demonstrations that nanoparticle (NP) and DNA scaffolding can enhance enzyme activity suggest new avenues toward this. A modular architecture consisting of a DNA cage displaying semiconductor quantum dots (QDs) that, in turn, ratiometrically display the organophosphate hydrolase phosphotriesterase (PTE) was utilized as a model system. Increasing DNA cage concentration relative to QD-PTE and creating a dense composite enhanced PTE rates up to 12.5-fold, suggesting strong synergy between the NP and DNA components; this putatively arises from increased enzymatic stability and alleviation of its rate-limiting step. Such bioinorganic composites may offer new scaffolding approaches for synthetic biology
%U https://pubs.acs.org/doi/10.1021/acsanm.8b00933