%0 Journal Article
%T A Single Enzyme Oxidative ¡°Cascade¡± via a Dual-Functional Galactose Oxidase
%J -
%D 2018
%R https://doi.org/10.1021/acscatal.8b00043
%X The galactose oxidase (GOase) M3-5 variant, previously engineered for enantioselective oxidation of (R)-secondary alcohols, is now shown to catalyze the sequential four-electron oxidation of substituted benzylic and heteroaromatic benzylic alcohols to the corresponding carboxylic acids via the intermediate aldehyde. Aldehyde oxidation has been shown to occur on the hydrated (gem-diol) form of the aldehyde, and hence the activity of this second oxidation step is primarily determined by the effects of substituents on the aromatic ring. The demonstration of GOase for ¡°through oxidation¡± of alcohols to carboxylic acids represents a fusion of the activities of two distinct copper radical oxidases (galactose oxidase and glyoxal oxidase) into a single enzyme sequence with potential applications as an abbreviated oxidative cascade
%U https://pubs.acs.org/doi/10.1021/acscatal.8b00043