%0 Journal Article
%T Oligonucleotide Determination via Peptide Nucleic Acid Macromolecular Imprinting in an Electropolymerized CG-Rich Artificial Oligomer Analogue
%J -
%D 2018
%R https://doi.org/10.1021/acsami.8b09296
%X We devised and fabricated a chemosensor for determination of the genetically relevant 5¡ä-GCGGCGGC-3¡ä (G = guanine; C = cytosine) oligonucleotide. For that, we simultaneously electrosynthesized and electrode-immobilized a sequence-defined octakis(2,2¡ä-bithien-5-yl) DNA hybridizing probe using both a ¡°macromolecular imprinting in polymer strategy¡± and a sequence-programmable peptide nucleic acid (PNA) template. With electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) transductions under stagnant-solution and flow injection analysis (FIA) conditions, respectively, we determined the above oligonucleotide with 200-pM EIS limit of detection. With its EIS-determined apparent imprinting factor of ¡«4.0, the chemosensor was discriminative to both mismatched oligonucleotides and Dulbecco¡¯s modified Eagle¡¯s medium sample interferences
%U https://pubs.acs.org/doi/10.1021/acsami.8b09296