%0 Journal Article
%T Lipid-Modified Graphene-Transistor Biosensor for Monitoring Amyloid-¦Â Aggregation
%J -
%D 2018
%R https://doi.org/10.1021/acsami.8b01917
%X A graphene field-effect transistor (G-FET) with the spacious planar graphene surface can provide a large-area interface with cell membranes to serve as a platform for the study of cell membrane-related protein interactions. In this study, a G-FET device paved with a supported lipid bilayer (referred to as SLB/G-FET) was first used to monitor the catalytic hydrolysis of the SLB by phospholipase D. With excellent detection sensitivity, this G-FET was also modified with a ganglioside GM1-enriched SLB (GM1-SLB/G-FET) to detect cholera toxin B. Finally, the GM1-SLB/G-FET was employed to monitor amyloid-beta 40 (A¦Â40) aggregation. In the early nucleation stage of A¦Â40 aggregation, while no fluorescence was detectable with traditional thioflavin T (ThT) assay, the prominent electrical signals probed by GM1-SLB/G-FET demonstrate that the G-FET detection is more sensitive than the ThT assay. The comprehensive kinetic information during the A¦Â40 aggregation could be collected with a GM1-SLB/G-FET, especially covering the kinetics involved in the early stage of A¦Â40 aggregation. These experimental results suggest that SLB/G-FETs hold great potential as a powerful biomimetic sensor for versatile investigations of membrane-related protein functions and interaction kinetics
%U https://pubs.acs.org/doi/10.1021/acsami.8b01917