%0 Journal Article
%T TiO2 Nanolayer-Enhanced Fluorescence for Simultaneous Multiplex Mycotoxin Detection by Aptamer Microarrays on a Porous Silicon Surface
%J -
%D 2018
%R https://doi.org/10.1021/acsami.8b01431
%X A new aptamer microarray method on the TiO2每porous silicon (PSi) surface was developed to simultaneously screen multiplex mycotoxins. The TiO2 nanolayer on the surface of PSi can enhance the fluorescence intensity 14 times than that of the thermally oxidized PSi. The aptamer fluorescence signal recovery principle was performed on the TiO2每PSi surface by hybridization duplex strand DNA from the mycotoxin aptamer and antiaptamer, respectively, labeled with fluorescence dye and quencher. The aptamer microarray can simultaneously screen for multiplex mycotoxins with a dynamic linear detection range of 0.1每10 ng/mL for ochratoxin A (OTA), 0.01每10 ng/mL for aflatoxins B1 (AFB1), and 0.001每10 ng/mL for fumonisin B1 (FB1) and limits of detection of 15.4, 1.48, and 0.21 pg/mL for OTA, AFB1, and FB1, respectively. The newly developed method shows good specificity and recovery rates. This method can provide a simple, sensitive, and cost-efficient platform for simultaneous screening of multiplex mycotoxins and can be easily expanded to the other aptamer-based protocol
%U https://pubs.acs.org/doi/10.1021/acsami.8b01431