%0 Journal Article
%T The Development of Cytogenetic Maps for Malaria Mosquitoes
%A Gleb N. Artemov
%A Igor V. Sharakhov
%A Maria V. Sharakhova
%A Vladimir N. Stegniy
%J Insects | An Open Access Journal from MDPI
%D 2018
%R https://doi.org/10.3390/insects9030121
%X Abstract Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi. View Full-Tex
%K cytogenetic map
%K chromosome map
%K malaria mosquitoes
%K Anopheles
%K polytene chromosomes
%K Anopheles beklemishevi
%K chromosome map development
%K photomap
%K high-pressure technique
%K chromosome straightening
%U https://www.mdpi.com/2075-4450/9/3/121