%0 Journal Article
%T Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae
%A Ali Taheri
%A Anthony Kusalik
%A Irene Martin
%A Jason P Acker
%A Jo-Anne R Dillon
%A Nurul H Khan
%A Pauline Skiba
%A Rajinder P Parti
%A Stephanie Stefura
%A Sumudu R Perera
%J Antibiotics | An Open Access Journal from MDPI
%D 2018
%R https://doi.org/10.3390/antibiotics7030070
%X Abstract Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non- Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis. View Full-Tex
%K Neisseria gonorrhoeae
%K molecular diagnosis
%K RT-PCR
%K hydrogel
%U https://www.mdpi.com/2079-6382/7/3/70