%0 Journal Article
%T NO-Donor Nitrosyl Iron Complex with 2-Aminophenolyl Ligand Induces Apoptosis and Inhibits NF-百B Function in HeLa Cells
%A Alexei Terent＊ev
%A Anastasia Balakina
%A Galina Kozub
%A Natalia Sanina
%A Tatiana Kondrat＊eva
%A Tatiana Stupina
%J -
%D 2018
%R https://doi.org/10.3390/scipharm86040046
%X Abstract NO donating iron nitrosyl complex with 2-aminothiophenyl ligand ( 2-AmPh complex) was studied for its ability to cause cell death and affect nuclear factor kappa B (NF-百B) signaling. The complex inhibited viability of HeLa cells and induced cell death that was accompanied by loss of mitochondrial membrane potential and characteristic for apoptosis phosphatidylserine externalization. At IC50, 2-AmPh caused decrease in nuclear content of NF-百B p65 polypeptide and mRNA expression of NF-百B target genes encoding interleukin-8 and anti-apoptotic protein BIRC3. mRNA levels of interleukin-6 and anti-apoptotic protein BIRC2 encoding genes were not affected. Our data demonstrate that NO donating iron nitrosyl complex 2-AmPh can inhibit tumor cell viability and induce apoptosis that is preceded by impairment of NF-百B function and suppression of a subset of NF-百B target genes. View Full-Tex
%K iron nitrosyl complex
%K 2-aminothiophenyl
%K apoptosis
%K NF-百B
%K p65
%K IL6
%K IL8
%K BIRC2
%K BIRC3
%U https://www.mdpi.com/2218-0532/86/4/46