%0 Journal Article
%T A Novel Disulfide-Containing Polycationic Amphiphile: 1,28-Di[(cholest-5-en-3¦Â-yl)disulfanyl]-4,25-dioxo-3,8,12,17,21,26-hexaazaoctacosane Tetrahydrochloride
%A Elena V. Shmendel
%A Marina A. Zenkova
%A Mikhail A. Maslov
%A Nina G. Morozova
%A Pavel A. Puchkov
%A Valeria D. Andreeva
%J -
%D 2018
%R https://doi.org/10.3390/M981
%X Abstract The absence of highly effective delivery systems is a major challenge for gene therapy. Our work was aimed at the development of novel cationic liposomes possessing high transfection efficiency. For this purpose, a novel disulfide polycationic amphiphile 2S4 was synthesized. Cationic liposomes based on 2S4 and a helper lipid DOPE were formed by the thin film hydration method and exhibited effective plasmid DNA delivery into the HEK293 cells, with a transfection activity superior to that of the commercial agent Lipofectamine £¿ 2000. Our results suggest that the polycationic amphiphile 2S4 is a promising candidate for in vitro nucleic acid delivery. View Full-Tex
%K redox-sensitive
%K disulfide linker
%K gemini amphiphiles
%K cationic liposomes
%K gene therapy
%U https://www.mdpi.com/1422-8599/2018/1/M981