%0 Journal Article %T Identification of Potential Plasma Biomarkers for Abdominal Aortic Aneurysm Using Tandem Mass Tag Quantitative Proteomics %A Anders E. Henriksson %A Carina Sihlbom %A Dan Bylund %A J£¿rgen Bergstr£¿m %A Markus Lindqvist %J - %D 2018 %R https://doi.org/10.3390/proteomes6040043 %X Abstract Plasma biomarkers that identify abdominal aortic aneurysm (AAA) rupture risk would greatly assist in stratifying patients with small aneurysms. Identification of such biomarkers has hitherto been unsuccessful over a range of studies using different methods. The present study used an alternative proteomic approach to find new, potential plasma AAA biomarker candidates. Pre-fractionated plasma samples from twelve patients with AAA and eight matched controls without aneurysm were analyzed by mass spectrometry applying a tandem mass tag (TMT) technique. Eight proteins were differentially regulated in patients compared to controls, including decreased levels of the enzyme bleomycin hydrolase. The down-regulation of this enzyme was confirmed in an extended validation study using an enzyme-linked immunosorbent assay (ELISA). The TMT-based proteomic approach thus identified novel potential plasma biomarkers for AAA. View Full-Tex %K aortic aneurysm %K biomarker %K bleomycin hydrolase %K clinical proteomics %K mass spectrometry %K proteomics %U https://www.mdpi.com/2227-7382/6/4/43