%0 Journal Article
%T Complementation Studies of Bacteriophage ¦Ë O Amber Mutants by Allelic Forms of O Expressed from Plasmid, and O-P Interaction Phenotypes
%A Connie Hayes
%A Karthic Rajamanickam
%A Sidney Hayes
%J Antibiotics | An Open Access Journal from MDPI
%D 2018
%R https://doi.org/10.3390/antibiotics7020031
%X Abstract ¦Ë genes O and P are required for replication initiation from the bacteriophage ¦Ë origin site, ori¦Ë, located within gene O. Questions have persisted for years about whether O-defects can indeed be complemented in trans. We show the effect of original null mutations in O and the influence of four origin mutations (three are in-frame deletions and one is a point mutation) on complementation. This is the first demonstration that O proteins with internal deletions can complement for O activity, and that expression of the N-terminal portion of gene P can completely prevent O complementation. We show that O-P co-expression can limit the lethal effect of P on cell growth. We explore the influence of the contiguous small RNA OOP on O complementation and P-lethality. View Full-Tex
%K P interactions on cell growth and O activity
%U https://www.mdpi.com/2079-6382/7/2/31