%0 Journal Article
%T Riboregulated toehold-gated gRNA for programmable CRISPR¨CCas9 function
%J -
%D 2018
%R https://doi.org/10.1038/s41589-018-0186-1
%X Predictable control over gene expression is essential to elicit desired synthetic cellular phenotypes. Although CRISPR¨CCas9 offers a simple RNA-guided method for targeted transcriptional control, it lacks the ability to integrate endogenous cellular information for efficient signal processing. Here, we present a new class of riboregulators termed toehold-gated gRNA (thgRNA) by integrating toehold riboswitches into sgRNA scaffolds, and demonstrate their programmability for multiplexed regulation in Escherichia coli with minimal cross-talks
%U https://www.nature.com/articles/s41589-018-0186-1