%0 Journal Article %T A chromatin integration labelling method enables epigenomic profiling with lower input %J - %D 2018 %R https://doi.org/10.1038/s41556-018-0248-3 %X Chromatin plays a crucial role in gene regulation, and chromatin immunoprecipitation followed by sequencing (ChIP每seq) has been the standard technique for examining protein每DNA interactions across the whole genome. However, it is difficult to obtain epigenomic information from limited numbers of cells by ChIP每seq because of sample loss during chromatin preparation and inefficient immunoprecipitation. In this study, we established an immunoprecipitation-free epigenomic profiling method named chromatin integration labelling (ChIL), which enables the amplification of genomic sequences closely associated with the target molecules before cell lysis. Using ChIL followed by sequencing (ChIL每seq), we reliably detected the distributions of histone modifications and DNA-binding factors in 100每1,000 cells. In addition, ChIL每seq successfully detected genomic regions associated with histone marks at the single-cell level. Thus, ChIL每seq offers an alternative method to ChIP每seq for epigenomic profiling using small numbers of cells, in particular, those attached to culture plates and after immunofluorescence %U https://www.nature.com/articles/s41556-018-0248-3