%0 Journal Article
%T Reliability of human cortical organoid generation
%J -
%D 2018
%R https://doi.org/10.1038/s41592-018-0255-0
%X The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling
%U https://www.nature.com/articles/s41592-018-0255-0