%0 Journal Article
%T Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution
%J -
%D 2019
%R https://doi.org/10.1038/s41592-019-0401-3
%X We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations
%U https://www.nature.com/articles/s41592-019-0401-3