%0 Journal Article
%T In vivo profiling of metastatic double knockouts through CRISPR¨CCpf1 screens
%J -
%D 2019
%R https://doi.org/10.1038/s41592-019-0371-5
%X Systematic investigation of the genetic interactions that influence metastatic potential has been challenging. Here we developed massively parallel CRISPR¨CCpf1/Cas12a crRNA array profiling (MCAP), an approach for combinatorial interrogation of double knockouts in vivo. We designed an MCAP library of 11,934£¿arrays targeting 325£¿pairwise combinations of genes implicated in metastasis. By assessing the metastatic potential of the double knockouts in mice, we unveiled a quantitative landscape of genetic interactions that drive metastasis
%U https://www.nature.com/articles/s41592-019-0371-5