%0 Journal Article
%T High-throughput identification of dominant negative polypeptides in yeast
%J -
%D 2019
%R https://doi.org/10.1038/s41592-019-0368-0
%X Dominant negative polypeptides can inhibit protein function by binding to a wild-type subunit or by titrating a ligand. Here we use high-throughput sequencing of libraries composed of fragments of yeast genes to identify polypeptides that act in a dominant negative manner, in that they are depleted during cell growth. The method can uncover numerous inhibitory polypeptides for a protein and thereby define small inhibitory regions, even pinpointing individual residues with critical functional roles
%U https://www.nature.com/articles/s41592-019-0368-0