%0 Journal Article
%T A photocleavable surfactant for top-down proteomics
%J -
%D 2019
%R https://doi.org/10.1038/s41592-019-0391-1
%X We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS¨Cpolyacrylamide gel electrophoresis
%U https://www.nature.com/articles/s41592-019-0391-1