%0 Journal Article
%T Genome editing in plants by engineered CRISPR¨CCas9 recognizing NG PAM
%J -
%D 2018
%R https://doi.org/10.1038/s41477-018-0321-8
%X Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5¡ä end of the target sequence
%U https://www.nature.com/articles/s41477-018-0321-8