%0 Journal Article
%T Jasmonate-mediated wound signalling promotes plant regeneration
%J -
%D 2019
%R https://doi.org/10.1038/s41477-019-0408-x
%X Wounding is the first event triggering regeneration1,2,3,4. However, the molecular basis of wound signalling pathways in plant regeneration is largely unclear. We previously established a method to study de novo root regeneration (DNRR) in Arabidopsis thaliana5,6, which provides a platform for analysing wounding. During DNRR, auxin is biosynthesized after leaf detachment and promotes cell fate transition to form the root primordium5,6,7. Here, we show that jasmonates (JAs) serve as a wound signal during DNRR. Within 2£¿h of leaf detachment, JA is produced in leaf explants and activates ETHYLENE RESPONSE FACTOR109 (ERF109). ERF109 upregulates ANTHRANILATE SYNTHASE ¦Á1 (ASA1)¡ªa tryptophan biosynthesis gene in the auxin production pathway8,9,10¡ªdependent on the pre-deposition of SET DOMAIN GROUP8 (SDG8)-mediated histone H3 lysine 36 trimethylation (H3K36me3)11 on the ASA1 locus. After 2£¿h, ERF109 activity is inhibited by direct interaction with JASMONATE-ZIM-DOMAIN (JAZ) proteins to prevent hypersensitivity to wounding. Our results suggest that a dynamic JA wave cooperates with histone methylation to upregulate a pulse of auxin production and promote DNRR in response to wounding
%U https://www.nature.com/articles/s41477-019-0408-x