%0 Journal Article
%T CRISPR RNA-guided autonomous delivery of Cas9
%J -
%D 2018
%R https://doi.org/10.1038/s41594-018-0173-y
%X Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors to specified genetic addresses. High-resolution structures of this protein and its associated CRISPR RNA guide explain the molecular mechanisms of CRISPR-RNA-guided DNA recognition and provide a molecular blueprint that has facilitated structure-guided functional remodeling. Here we retrace events that led from early efforts to understand the central role of Cas9 in CRISPR-mediated adaptive immunity to contemporary efforts aimed at developing and deploying this enzyme for programmable genetic editing
%U https://www.nature.com/articles/s41594-018-0173-y