%0 Journal Article
%T Purification and Characterization of Protease From Bacillus sp. TBRSN- 1
%J -
%D 2013
%R https://doi.org/10.22146/ijbiotech.7878 https://doi.org/10.22146/ijbiotech.7878
%X Potato Cyst Nematode (PCN), Globodera rostochiensis, is one of the important potato¡¯s pests and causedeconomic looses up to 70% in the several centrals of potato plantations in Indonesia. PCN¡¯s shell componentof egg shell containing chitin (inner layer) and viteline/ protein (outer layer). The purpose of this researchwas to purify of protease Bacillus sp. TBRSN-1, isolate from tomato¡¯s rhizosfer in Yogyakarta province. Thepurifi ed protease could be used for cutting the life cycle of PCN. Results showed that Bacillus sp. TBRSN-1could produce extracellular protease and purifi cation using DEAE-cellulose ion-exchange chromatographyand Sephacryl S-300 gel fi ltration chromatography resulted in specifi c activity 4.31 fold and 1.68% recovery.Analysing using SDS-PAGE 12.5% and molecular weight 48.1 kDa. Km and Vmax values of the protease forcasein substrate were 7.83 mg/ml and 4.03 ¦Ìg/h, respectively. The optimum activity at the temperature30oC and pH 7.0. Keywords : protease, purifi cation, indigenous Bacillus sp. TBRSN-
%K protease
%K purification
%K indigenous Bacillus sp. TBRSN-1
%U https://jurnal.ugm.ac.id/ijbiotech/article/view/7878