%0 Journal Article
%T Selection of Phalaenopsis amabilis L. Blume Orchid Resistance to Hygromycin
%A Aziz Purwantoro
%A Endang Semiarti
%A Ixora Sartika Mercuriani
%A Seonghoe Jang
%A Sukarti Moeljopawiro
%J -
%D 2012
%R https://doi.org/10.22146/ijbiotech.16000 https://doi.org/10.22146/ijbiotech.16000
%X Examination of Phalaenopsis amabilis orchid resistance to hygromycin antibiotic is an important step to do prior to Agrobacterium-mediated genetic transformation in this orchids using Hygromycin phosphotransferase (HPT) gene as a selection marker in the T-DNA that harboring a desired gene to be transfered. We exposed the plant on hygromycin containing medium. The experiment was conducted using 6 weeks old P. amabilis protocorms. These protocorms were subcultured onto NP medium supplemented with various concentration of Hygromycin (0, 5, 10, 20, 1nd 40 mg/l). The number of survival protocorms were examined every week for 4 weeks after subcultured (WAS). The resistancy of hygromycin was calculated as ratio of death protocorms per total protocorms). The result showed that 10 mg/l hygromycin with 1 weeks of application caused death close to LD 50. This data indicate that P. amabilis resistance to hygromycin treatment on the appropriate concentration 10 mg/l, and this concentration can be used for other purposes in orchid system
%K hygromycin
%K resistance
%K LD50
%K P. amabilis
%K protocorm
%U https://jurnal.ugm.ac.id/ijbiotech/article/view/16000