%0 Journal Article
%T A Novel Approach to Assess the Dynamics of Extra-Chromosomal Circular Ribosomal DNA in Human Cells
%A Forbes E Katz V
%A Husko C
%A Joerg R Leheste
%A Miceli A
%A Ramos RL
%A Torres G
%J -
%D 2015
%R 10.18314/gjbs.v1i1.21
%X Several nutrient-signaling pathways that extend life span have been described in model organisms.£¿£¿£¿ Thus, parallel and redundant signaling pathways that are similar across species might be subject to experimental£¿£¿£¿ manipulation. Here, we develop a PCR-based technique for testing the hypothesis that mitotic£¿£¿£¿ accumulation of extra-chromosomal ribosomal DNA circles might also determine life span in£¿£¿£¿ human cells. Using resveratrol, a phytochemical that counters age-related signs, we find treatment-dependent£¿£¿£¿ subcellular accumulations of extra-chromosomal 5S ribosomal DNA in human cell lines. These£¿£¿£¿ data suggest an association between DNA circles and intrinsic aging and demonstrate the utility of a PCRbased£¿£¿£¿ technique for studying the accumulation of dysfunctional molecules that promote senescence
%U https://www.gratisoa.org/journals/index.php/GJBS/article/view/21