%0 Journal Article
%T Alteration in transforming growth factor-¦Â receptor expression in gallbladder disease: implications of chronic cholelithiasis and chronic Salmonella typhi infection
%A Vijayashree Nayak
%A Yatindra Vaidya
%A Yogesh D. Walawalkar
%J -
%D 2016
%R https://doi.org/10.4081/gi.2016.6623
%X Gallbladder cancer prevalence is ever increasing with Salmonella typhi chronic infection being one of the predisposing factors. Altered ratios or expression of transforming growth factor-¦Â (TGF-¦Â) receptors and changes in its function are associated with loss in anti-proliferative effects of TGF-¦Â and cancer progression. Using reverse transcriptase polymerase chain reaction we monitor any changes in TGF-¦Â receptor gene expression. We simultaneously screen for S. typhi within the samples. From 73 patients undergoing cholecystectomy 39-50% had significant expression (P<0.05) of TGF-¦Â receptor (T¦ÂR)- I and T¦ÂR-II during chronic cholelithiasis as compared to the remaining 19-23% with acute chronic cholelithiasis. There was no significant increase in T¦ÂR-III receptor expression. Patient¡¯s positive for S. typhi (7/73) did not show any significant changes in expression of these receptors, thus indicating no direct relation in regulating the host TGF¦Â-signaling pathway. Further analysis on expression of downstream Smad components revealed that patients with up-regulated TGF¦Â receptor expression show >2-fold increase in the RSmads and Co-Smads with a >2-fold decrease in I-Smads. Thus gain of T¦ÂR-I and II expression in epithelial cells of the gallbladder was associated with chronic inflammatory stages of the gallbladder disease.
%U https://www.pagepress.org/journals/index.php/gi/article/view/6623