%0 Journal Article
%T Acute monocytic leukemia diagnosed by flow cytometry includes acute myeloid leukemias with weakly or faintly positive non-specific esterase staining
%A Ayano Mori
%A Hiroko Tsunemine
%A Miho Sasaki
%A Taiichi Kodaka
%A Takae Goka
%A Takayuki Takahashi
%A Toshiharu Saitoh
%A Yumi Aoyama
%A Yuriko Zushi
%A Yuta Goto
%J -
%D 2016
%R https://doi.org/10.4081/hr.2018.7435
%X A diagnosis of acute monocytic leukemia(AML-M5) based on ¦Á-naphthyl butyrateesterase (¦Á-NB) staining has some problems,because AML-M5 leukemic cells often showweak or faint positivity on ¦Á-NB staining. Inthese situations, some cases of AML-M5tend to be misdiagnosed as AML-M0. Therefore, we evaluated the significance ofweak or faint ¦Á-NB staining in AML-M5diagnosed by flow cytometry (FCM). Nineteen AML cases in which leukemic cellswere negative for naphthol AS-D chloroac-etate esterase staining were studied. ForFCM, we defined leukemic cells as having amonocytic nature when more than 10% ofthe leukemic cells were positive for at leastone of the following antigens: CD4, CD11c,CD14, and CD64. The monocytic naturedetermined by FCM was consistent with pos-itive or weak positivity on ¦Á-NB staining. Five of 6 cases in which leukemic cellsexhibited faint positivity for ¦Á-NB stainingcould be diagnosed as AML-M5 by FCM,while negative ¦Á-NB staining was consistentwith a diagnosis of AML-M0. These resultssuggest that AML-M5 should be taken intoconsideration even when leukemic cells arefaintly positive for ¦Á-NB staining.
%U https://www.pagepress.org/journals/index.php/hr/article/view/7435