%0 Journal Article
%T Embryogenic Callus Induction and Efficient Plant Regeneration in Three Varieties of Soybean (Glycine max)
%A Bakul Bhattacharjee
%A M Monzur Hossain
%A Md Munir Hossain
%A Nazrul Islam
%A SM Shahinul Islam
%A Touhidul Islam
%J Plant Tissue Culture and Biotechnology
%D 2017
%R https://doi.org/10.3329/ptcb.v27i1.35011
%X An efficient callus induction and plant regeneration system has been developed using three local soybean (Glycine max L.) varieties. All the varieties showed good callusing (78.30 ©\ 88.80%) from shoot tip (ST) in MS + 3.0 mg/l 2,4©\D + 1.0 mg/l BAP. Best callus induction and embryo formation were recorded in T5 for BS©\6 as 88.80 and 81.20%, respectively from ST. In case of cotyledonary node (CN) the maximum callus induction (82.40%) and embryo formation (74.20%) were recorded also in T5 for BS©\6. Highest frequency (79.40%) of plant regeneration was recorded where ST were used and cultured in MS supplemented with 2.5 mg/l BAP + 1.0 mg/l NAA as well as 76.30% from CN in BS©\6. The length of shoot was observed 5.32 and 4.62 cm, respectively from ST and CN for BS©\6 with the same medium composition. It was observed that half strength of MS + 2.0 mg/l IBA showed best rooting (9.04). Among the genotypes BS©\6 proved to be best explants than ST that exhibited better performance on callus induction and green plant regeneration for all parameters. Plant Tissue Cult. & Biotech. 27(1): 41-50, 2017 (June)
%K Cotyledonary node
%K Embryogenic calli
%K Regeneration
%K Somatic embryogenesis
%K Glycine max
%U https://www.banglajol.info/index.php/PTCB/article/view/35011