%0 Journal Article
%T Effect of Methyl Jasmonate and Mannitol Application on Growth and Eugenol Content in Callus Cultures of Carnation
%A Moemen S Hanafy
%A Mohamed A Matter
%A Usama I Aly
%J Plant Tissue Culture and Biotechnology
%D 2017
%R https://doi.org/10.3329/ptcb.v27i2.35028
%X A protocol for callus induction from leaves of carnation is established. MS fortified with 2.0 mg /l 2,4©\D and 0.5 mg/l Kn was found to be the appropriate medium for producing callus cultures. Methyl jasmonate at 20 ¦ÌM concentration showed the highest growth rate (98.21 mg/day) while mannitol concentrations at 2% gave the peak growth rate (97.1 mg/day).The highest content of eugenol (14.65 ¦Ìg/100 g calli) was recorded with medium containing 20 ¦ÌM methyl jasmonate followed by 8.16 ¦Ìg/100 g calli with 10 ¦ÌM methyl jasmonate. In presence of 0.5% mannitol, maximum eugenol content (18.00 ¦Ìg/100 g calli) was obtained. Plant Tissue Cult. & Biotech. 27(2): 227-240, 2017 (December)
%K Dianthus caryophyllus
%K Methyl Jasmonate
%K Mannitol
%K Eugenol
%K HPLC
%U https://www.banglajol.info/index.php/PTCB/article/view/35028