%0 Journal Article
%T Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice
%J -
%D 2018
%X A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner
%K Rice
%K OsWD1
%K Overexpression
%K Plant Height
%K Grain Size
%K Gibberellin Acid
%U http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=218&doi=10.11648/j.ajaf.20180602.11