%0 Journal Article
%T Optimum Immobilization of Candida Antartica B Lipase on Natural Silica by RSM
%J American Journal of Chemical Engineering
%@ 2330-8613
%D 2017
%X The immobilization of the lipase of Candida antartica B (LCAB) by adsorption on a natural silica support carried out to develop the adsorbent local supports. Immobilization conditions and characterization of the immobilized enzyme were investigated. Response surface methodology (RSM) and 3-level¨C3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (5-25 hour), immobilization temperature (25-45ˇăC), and enzyme/support ratio (0.1-0.5, w/w), on yield of lipase immobilization on the support. The optimum immobilization conditions were as follows: immobilization time 18 hours, immobilization temperature 20ˇăC, and enzyme / support ratio 0.5 (w/w); with a yield immobilization of 56,13 mg / g. The immobilization lipase shows hydrolytic and synthesis satisfactory activity.
%K Immobilization
%K Lipase
%K Natural Silica Support
%K RSM
%U http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=224&doi=10.11648/j.ajche.20170503.13