%0 Journal Article
%T Application of CLARITY to Investigate the 3D Architecture of 14-3-3 Zeta Protein in AD
%J -
%D 2017
%X CLARITY (Clear Lipid¨Cexchanged Acrylamide¨Chybridized Rigid Imaging/ Immunostaining/ in situ¨Chybridization¨Ccompatible Tissue hydrogel) is a powerful, innovative, whole brain-clearing technology, and it has been successfully combined with the immunofluorescence staining to achieve the 3D visualization of some proteins or cells in mm-thick brain tissue or even the intact brains. These 3D information help to gain deeper understanding on the pathologic mechanism of some neuronal diseases (for example Parkinson and Alzheimer). 14-3-3 zeta is a highly-expressed protein in Alzheimer¡¯s disease (AD) brain, which was closely related with the formation of Tau aggregation and neurofibrillary tangles. However, little useful information has been available concerning the 3D architecture of 14-3-3 zeta in AD disease. In this paper, the transgenic AD mice were used and the 1mm-thick brain slices were passively clarified. Immunofluorescence staining results showed that different from the control group, 14-3-3 zeta was mainly present around the hippocampus in AD mice. Additionally, the morphology of 14-3-3 zeta protein was filamentous with different lengths. This result will be helpful for exploring the in vivo role of 14-3-3 zeta protein during the progression of AD.
%K AD
%K CLARITY
%K 14-3-Zeta Protein
%K Immunofluorescence
%U http://www.sciencepublishinggroup.com/journal/paperinfo?journalid=259&doi=10.11648/j.ijbse.20170506.12