%0 Journal Article
%T Expression of Filaggrin and its Degradation Products in Human Skin Following Erythemal Doses of Ultraviolet B Irradiation
%A Jacob P. Thyssen
%A Lone Skov
%A Sanja Kezic
%A Steffen Heegaard
%A Stine Simonsen
%J -
%@ 1651-2057
%D 2017
%R 10.2340/00015555-2662
%X Epidermal filaggrin level is affected by ultraviolet B irradiation in animal and experimental models. This study examined the effect of ultraviolet B irradiation on epidermal filaggrin and natural moisturizing factors in vivo in healthy adults (n£¿=£¿22). Participants were irradiated with 2 minimal erythema doses of ultraviolet B on the skin. Biopsies and tape strips were collected from skin irradiated 24 and 72 h earlier and from non-irradiated skin (control). Real-time quantitative PCR on skin biopsies showed significantly reduced profilaggrin mRNA expression 24 h after irradiation (mean relative mRNA expression£¿¡À£¿standard deviation: control, 3.86£¿¡À£¿2.06 vs. 24 h, 1.52£¿¡À£¿0.640; p£¿=£¿0.02; n£¿=£¿8). Immunohistochemistry showed aberrant spatial distribution of filaggrin protein 72 h after irradiation (n£¿=£¿3). High-pressure liquid chromatography of tape extracts showed no change in mean total natural moisturizing factor levels after irradiation, but mean trans-urocanic acid was significantly reduced, as expected (n£¿=£¿8). In conclusion, erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo.
%U http://www.medicaljournals.se/acta/content/abstract/10.2340/00015555-2662