%0 Journal Article
%T 耻垢分枝杆菌产乳酸氧化酶条件的研究
%J -
%D 2006
%X 摘要 以1株耻垢分枝杆菌(Mycobacterium smegmatis)为出发菌株,对其产乳酸氧化酶的培养基和发酵条件进行研究.结果表明,培养基中添加3%甘油,0.4%蛋白胨,1%DL-乳酸,0.005%VB1,0.05%KH2PO4·H2O,0.05%MgSO4·7H2O,0.01%硫酸亚铁胺有助于产酶;优化培养条件为:250 mL三角瓶中装液量50 mL,起始pH7.2,培养温度37℃,静置培养4 d,乳酸氧化酶平均酶活为65.4 U/mL
%U http://sf1970.cnif.cn/CN/abstract/abstract13276.shtml