%0 Journal Article
%T Expression of Difficult-to-Express Proteins, Human IL-12 and IFN-<i>¦Ã</i>
%A Yoshihito Hosaka
%A Shun Matsutani
%A Shinya Kawate
%A Kei Itoh
%A Atsuko Miura
%A Yukaze Mizoura
%A Sayumi Yamada
%A Seiya Uemura
%A Hiroshi Konno
%A Ewa Grave
%A Hideki Wakui
%A Hideaki Itoh
%J American Journal of Molecular Biology
%P 29-37
%@ 2161-6663
%D 2021
%I Scientific Research Publishing
%R 10.4236/ajmb.2021.112003
%X It is known to be that lactic acid bacteria induce the IL-12. IL-12 activates NK cells and promotes the production of IFN-<em>¦Ã</em>. IFN-<em>¦Ã</em> activates macrophages, resulting in enhanced phagocytosis and bactericidal activity. We have been investigating fermented foods that activate the immune function. For that purpose, a specific antibody is required. We tried to express IL-12p35 by the usual method, but IL-12p35 was not expressed at all. In the present study, we constructed, purified human IL-12p35 and obtained a specific antibody against IL-12p35. We also purified human IFN-<em>¦Ã</em> and obtained specific antibody against IFN-<em>¦Ã</em>. We have established a method for expressing poorly expressed proteins. The method we have established can be applied to the purification of poorly expressed proteins and antibody production.
%K Difficult-to-Express Proteins
%K IL-12
%K IFN-&lt
%K i&gt
%K ¦Ã&lt
%K /i&gt
%K Purification
%K Antibody Production
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=107559