%0 Journal Article
%T Detection of Campylobacter sp. from Poultry Feces in Ouagadougou, Burkina Faso
%A Ass¨¨ta Kagamb¨¨ga
%A Alexandre Thibodeau
%A Daniel K. Soro
%A Nicolas Barro
%A Philippe Fravalo
%J Food and Nutrition Sciences
%P 107-114
%@ 2157-9458
%D 2021
%I Scientific Research Publishing
%R 10.4236/fns.2021.122009
%X Background: Campylobacter contamination in
poultry and poultry product has been reported worldwide. The present study aims
to determine the prevalence of Campylobacter in poultry feces using selective enrichment Bolton broth and multiplex PCR. Method: Two methods were used in this study: the first was direct plating of poultry feces
into mCCDA agar plates. The second, three antibiotics were used at different concentrations
to add in Bolton broth supplemented. These antibiotics were Rifampicin (Oxoid, Nepean,
Ontario) with 10 mg/L, colistin
(Oxoid, Nepean, Ontario) with 1 mg/mL
and 2 mg/mL;
trimethoprim (Oxoid, Nepean, Ontario) with 10 mg/L. The colonies with typical campylobacter morphology on blood agar (little, red and ring colonies) were further identified to
the species level by multiplex polymerase chain reaction (PCR). Results: The addition of colistin (2 mg/mL)
to the Bolton broth with selective supplements enhanced the selective isolation
of