%0 Journal Article
%T 黑果腺肋花楸花青素提取工艺研究<br>Study on the Extraction Technology of Anthocyanin from Sorbus melanocarpa
%A 王一涵
%A 吴镝
%A 张铭津
%A 张帆
%A 王薇
%J Botanical Research
%P 56-62
%@ 2168-5673
%D 2021
%I Hans Publishing
%R 10.12677/BR.2021.101008
%X <div style=\"text-align:justify;\">
	<span style=\"font-size:14px;line-height:1.5;\">黑果腺肋花楸果实中富含大量营养物质，例如膳食纤维、糖类、蛋白质、多酚类化合物等，其中最重要的物质是酚类物质。多酚类化合物如酚酸、原花青素、花青素、黄酮醇等含量丰富，且与黑果腺肋花楸的生物活性密切相关。其中花青素占主导地位，约占总酚的25%，可保护机体免受自由基损伤，有防癌、护肝等多种作用。本文为研究黑果腺肋花楸花青素的提取工艺，进一步提高黑果腺肋花楸花青素的提取效率，利用正交实验的方式优化超声波辅助提取黑果腺肋花楸花青素。具体的提取方法是以黑果腺肋花楸果实作为原料，应用乙醇作为提取溶剂，通过超声辅助提取的方法提取花青素，在单因素基础上，考察乙醇体积分数、液料比、提取时间、超声功率对黑果腺肋花楸花青素提取率的影响，利用L9(34)正交试验确定最佳提取工艺条件。研究结果表明，黑果腺肋花楸花青素的最佳提取工艺为乙醇体积分数为65%、液料比为6:1 (mL/g)、提取时间为20 min、超声功率为200 W，在此条件下花青素的提取率为(33.5 ± 1.2 mg/g)。本文研究发现超声波辅助提取紫黑果腺肋花楸青素效率比较高、用时少。超声波使样品与溶剂之间产生空化作用，促进样品分散，从而增大样品与溶剂间的接触面积，提高有效物质的溶出，进而提高提取率。超声波辅助提取法较传统的溶剂提取法操作简单、快速，具有一定的推广价值和广泛的应用前景。<br />
The fruit of Sorbus melanocarpa is rich in a large number of nutrients, such as dietary fiber, Car-bohydrates, protein, and polyphenolic compounds. The most important substances are phenols. Polyphenolic compounds such as phenolic acids, proanthocyanidins, anthocyanins, flavonols, etc. are rich in content, and are closely related to the biological activity of Sorbus melanocarpa. Among them, anthocyanins dominate, accounting for about 25% of the total phenols. They can protect the body from free radical damage, and have many functions such as preventing cancer and protecting the liver. Therefore, the fruit of Aronia melanocarpa also has a certain health effect. This article is to study the extraction process of S. melanocarpa anthocyanins, further improve the extraction efficiency of S. melanocarpa anthocyanins, and optimize the ultrasonic-assisted extraction of anthocyanins from S. melanocarpa by orthogonal experiments. The specific extraction method is based on Sorbus melanocarpa fruit as raw material, ethanol is used as extraction solvent, and anthocyanins are extracted by ultrasonic-assisted extraction. On the basis of single factor, the ethanol volume fraction, liquid-to-material ratio, and extraction time are investigated. The effect of ultrasonic power on the extraction rate of anthocyanin from Sorbus melanocarpa, the L9 (34) orthogonal test was used to determine the optimal extraction process conditions. The results of the study showed that the best extraction process for anthocyanins from S. melanocarpa was 65% ethanol volume fraction, 6:1 liquid-to-solid ratio (mL/g), extraction time 20 min, and ultrasonic power 200 W. Under these conditions, the extraction rate of anthocyanins was (33.5 ± 1.2 mg/g). The research in this paper found that the ultrasonic assisted extraction of aronia cyanidin is more efficient and takes less time. Ultrasonic waves cause cavitation between the sample and the
%K 黑果腺肋花楸，花青素，提取工艺，正交优化<br>Sorbus melanocarpa
%K Anthocyanin
%K Extraction Process
%K Orthogonal Optimization
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=39746