%0 Journal Article
%T 产脂肪酶菌株的筛选、鉴定与产酶条件优化<br>The Screening and Identification of a Lipase-Producing Strain and Its Culture Condition Optimization
%A 黎小军
%A 谢莲萍
%A 刘建宏
%A 朱婷婷
%A 刘蓉
%J -
%D 2014
%X 以橄榄油为唯一碳源,采用平板变色圈法,从土壤中筛选到1株脂肪酶高产菌XYU-6,经生理生化试验鉴定及16S rDNA序列分析,XYU-6被鉴定为洋葱假单胞菌(Burkholderia cepacia).设计单因素试验,对其产酶条件进行优化,优化后的培养条件为:葡萄糖1.00；,蛋白胨2.00；,豆油1.00；,KH2 PO40.35；,K2 HPO4 0.15；,MgSO40.05；,初始pH值为7.0,培养温度30℃,培养48 h,酶活可达24.1U？mL-1,是优化前的1.65倍.<br>A lipase producing strain XYU-6 was isolated from the soil by the method of flat discolor circle with olive oil as sole carbon source.XYU-6 was identified as Burkholderia cepacia by 16S rDNA sequencing and its biochemical and physiological characteristics.The optimal conditions,glucose 1.0%,peptone 2.0%,soya-bean oil 1%,KH2PO4 0.35%,K2HPO4 0.15% and MgSO4 0.05% were obtained through one-factor experimental design.Furthermore,the highest lipase activity 24.1 U？mLwas achieved under the optimum conditions with initial pH 7.0,culture temperature 30 ℃ and culture time 48 h.These results indicated that the lipase activity was improved 1.65 fold compared to the original conditions
%K 脂肪酶
%K 洋葱假单胞菌
%K 筛选
%K 鉴定
%K 优化<br>脂肪酶 洋葱假单胞菌 筛选 鉴定 优化
%K 脂肪酶 洋葱假单胞菌 筛选 鉴定 优化
%K 脂肪酶 洋葱假单胞菌 筛选 鉴定 优化
%K 脂肪酶 洋葱假单胞菌 筛选 鉴定 优化
%K 脂肪酶 洋葱假单胞菌 筛选 鉴定 优化
%U http://lkxb.jxnu.edu.cn//oa/darticle.aspx?type=view&id=20140104